GFP western blot from Drosophila embryos

IndiraShukla Indira_Shukla at brown.edu
Thu May 25 14:45:29 EST 2000


I am also facing the same problem, lots of non-specific band. After
reading your post, I will switch to Roche's Ab. What dilution of these ab
do you use? 

In article <391BC62D.FB0CDB97 at nowhere.com>, hase at nowhere.com wrote:

> We use the monoclonal abs mix from Boehringer Mannheim (=Roche these
> days...) and
> never had a problem. I know that the abs that Clontech sells do not work
> (at least in our hands)
> 
> Daniel
> 
> Sven Pfeiffer wrote:
> 
> > Hi there,
> >
> >         has anyone done Western blots with a GFP antibody from embryos?
> > My
> > own efforts are rather poor, lots of unspecific bands it seems. I am
> > looking for a clean antibody and a reliable protocol.
> >
> > Cheers
> >
> > Sven
> >
> > ********************************************************
> >
> > National Institute for Medical Research (N.I.M.R.)
> > Division of Mammalian Development
> > The Ridgeway, Mill Hill
> > London NW7 1AA
> > U.K.
> >
> > tel.: +44 (181) 959 3666 ext. 2017
> > fax : +44 (181) 913 8543
> >
> > ********************************************************
> >
> > ---






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