Error Prone PCR
Indira_Shukla at brown.edu
Thu May 25 15:27:03 EST 2000
In article <392C05BA.4710D250 at biocomp.unl.edu>, Chris LaRosa
<clarosa at biocomp.unl.edu> wrote:
> Simon Margerison wrote:
> > Hi
> > A colleague of mine is interested in performing some error prone pcr.
> > Does anyone know any good methods or reagents for this. Any help would
> > be appreciated, either by direct email to
> > ruth.featherstone at camb-antibody.co.uk
> > or on the board.
> Try using a higher concentration of dNTPs..... this will increase the
> errors. I know that a lower concentration of dNtps will give lower
> I am speculating here: limit the concentration of 3 of the dntps and
> increase the concentration of the remainder. This should cause more
> errors. How much so? Try a range of concentrations....
> I make these comments,, haveing never done such an experiment, so take
> them with a grain of salt.
Try using polymerase which has the proofreading part missing. Such enzymes
are sold commercially by various companies. I have used one such enzyme
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