crackling sequencing gels
rh at mblab.gla.ac.uk
Fri May 26 04:08:22 EST 2000
You really should get your news programme fixed. :-)
Ar you using Mozilla or Outlook. Both post as html sometimes.
In article <392D527F.6FA2E4A at mbio.aau.dk>, "T. Max M. Soegaard"
<tmms at mbio.aau.dk> wrote:
> <!doctype html public "-//w3c//dtd html 4.0 transitional//en">
> First: thanks for responding (and to you to Anna)
> <br>(I probably should have looked this up in C.P. or Maniatis first,
> but anyway:) Fixing the gel?
> <br>I don't. Hov do you fix the gel, exactly. Acetic acid and methanol...
> (that sounds like when I used to fix preparations of nuclei to make metaphase
> spreads).. What proportions of either do you use? How does one do
> <p>ParentE at dfo-mpo.gc.ca wrote:
> <blockquote TYPE=CITE>Hi Max.
> <p>What do you use to fix your gel? Acetic acid and methanol, will ensure
> <br>your gel won't crack. Plain ethanol work as well but your gel shrink
> quite a
> <p>hope this helps
> <p>Eric Parent
> <br>Maurice Lamontagne Institute
> <br>Fisheries and Oceans Canada
> <br>Invertebrates and Experimental Biology
> <br>850 route de la mer, P.O. box. 1000
> <br>Mont-Joli, Qc
> <br>G5H 3Z4
> <p>parente at dfo-mpo.gc.ca
> <br>> <a
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