crackling sequencing gels

Robert Hartley rh at mblab.gla.ac.uk
Fri May 26 04:08:22 EST 2000


You really should get your news programme fixed. :-)
Ar you using Mozilla or Outlook. Both post as html sometimes.

Bob

In article <392D527F.6FA2E4A at mbio.aau.dk>, "T. Max M. Soegaard"
<tmms at mbio.aau.dk> wrote:

> <!doctype html public "-//w3c//dtd html 4.0 transitional//en">
> <html>
> First: thanks for responding (and to you to Anna)
> <br>Second:
> <br>Ahemm...
> <br>(I probably should have looked this up in C.P. or Maniatis first,&nbsp;
> but anyway:)&nbsp; Fixing the gel?
> <br>I don't. Hov do you fix the gel, exactly. Acetic acid and methanol...
> (that sounds like when I used to fix preparations of nuclei to make metaphase
> spreads).. What proportions of either do you use?&nbsp; How does one do
> that?
> <p>-Max
> <p>ParentE at dfo-mpo.gc.ca wrote:
> <blockquote TYPE=CITE>Hi Max.
> <p>What do you use to fix your gel? Acetic acid and methanol, will ensure
> that
> <br>your gel won't crack. Plain ethanol work as well but your gel shrink
> quite a
> <br>lot.
> <p>hope this helps
> <p>Eric Parent
> <br>Maurice Lamontagne Institute
> <br>Fisheries and Oceans Canada
> <br>Invertebrates and Experimental Biology
> <br>850 route de la mer, P.O. box. 1000
> <br>Mont-Joli, Qc
> <br>Canada
> <br>G5H 3Z4
> <p>parente at dfo-mpo.gc.ca
> <br>> <a
href="http://www.qc.dfo-mpo.gc.ca/iml/fr/intro.htm">http://www.qc.dfo-mpo.gc.ca/iml/fr/intro.htm</a>
> <br>>
> <br>>
> <p>---</blockquote>
> </html>






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