Detection of low abundant genes in transgenic plants

Mathieu mathieu.ingouff at sgen.slu.se
Fri May 26 07:19:56 EST 2000


Hello there,

I have to analyse my transgenic plants (quite many) to see if there is
any over/under expression.
However i cannot detect anything with 20 ug total RNA but i know that
they are transgenic
The native gene cannot be detected by northern using 1 ug of mRNA so i
think that northern blotting is not sensitive enough

Is there a quick method to check that...i thought about using
quantitative PCR but it is quite painfull and time consuming.


Thank you for your help

Mathieu


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