Detection of low abundant genes in transgenic plants

Frederik Börnke ricky_boernke at gmx.net
Fri May 26 08:27:31 EST 2000



Mathieu schrieb:
> 
> Hello there,
> 
> I have to analyse my transgenic plants (quite many) to see if there is
> any over/under expression.
> However i cannot detect anything with 20 ug total RNA but i know that
> they are transgenic
> The native gene cannot be detected by northern using 1 ug of mRNA so i
> think that northern blotting is not sensitive enough
> 
> Is there a quick method to check that...i thought about using
> quantitative PCR but it is quite painfull and time consuming.
> 
> Thank you for your help
> 
> Mathieu


Hi,

what promoter, which plant? If it is 35S you should see something with
20 µg total RNA, at least in my experience. Are you sure your northern
is working? For screening northern blotting is definetly the method of
choice.

Ricky 
*******************************************************************

Frederik Boernke
Research Group of  Molecular Plant Physiology
Institute for Plant Genetics and Crop Plant Research (IPK)
Corrensstr. 3
06466 Gatersleben
Tel.  039482 -5 321
Fax. 039482 -5 515
http://www.ipk-gatersleben.de






More information about the Methods mailing list