Detection of low abundant genes in transgenic plants

Chris LaRosa clarosa at biocomp.unl.edu
Fri May 26 09:02:21 EST 2000



> Is there a quick method to check that...i thought about using
> quantitative PCR but it is quite painfull and time consuming.
> 
> Thank you for your help
> 
>
We had that problem trying to detect plant gamma glutamyl kinase in
plants.
First try collecting a a lot of poly a+ rna for northerns.   Some genes
require up to 20 ug of poly a to see on a northern.   Also consider
RNase protection assay which is more sensitive than northern.






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