Detection of low abundant genes in transgenic plants
clarosa at biocomp.unl.edu
Fri May 26 09:02:21 EST 2000
> Is there a quick method to check that...i thought about using
> quantitative PCR but it is quite painfull and time consuming.
> Thank you for your help
We had that problem trying to detect plant gamma glutamyl kinase in
First try collecting a a lot of poly a+ rna for northerns. Some genes
require up to 20 ug of poly a to see on a northern. Also consider
RNase protection assay which is more sensitive than northern.
More information about the Methods