PCR from genomic dna

Chris LaRosa clarosa at biocomp.unl.edu
Fri May 26 16:55:31 EST 2000



Kedar Vaidya wrote:
> 
> I have been trying to get a specific part of the dna amplifies by
> PCR..the primers seem pretty ok. they work when i pcr from plasmids but
> i get no product from the genomic dna...any suggestions?
> 
> --
> 
Look for dirty or degraded genomic dna template.  Can you pcr out using
say actin control primers?






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