PCR protocol

lors lorsNOloSPAM at mcafeemail.com.invalid
Mon May 29 09:28:11 EST 2000


Try to include 1M Betaine and/or 5% DMSO. These
compounds "melts" the DNA

:) Lars



In article <8gtdbo$2mh$1 at peque.uv.es>, "Jordi Pérez-Tur"
<jpereztur at ibv.csic.es> wrote:
>Hi,
>
>    I would be interested in protocols for amplifiying short
areas of the
>human genome (about 200bp) with a high GC content. So far we
have been quite
>unsuccesful with traditional profiles.
>
>    Thanks to all
>
>    Jordi Perez Tur
>    jpereztur at ibv.csic.es
>
>
>
>


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