PCR protocol

[Jordi Pérez-Tur]

Thanks Lars,

We have tried 5% DMSO with no luck, may be betaine....

Jordi

lors <lorsNOloSPAM at mcafeemail.com.invalid> escribió en el mensaje de
noticias 13e285c8.bb1a7002 at usw-ex0102-013.remarq.com...
> Try to include 1M Betaine and/or 5% DMSO. These
> compounds "melts" the DNA
>
> :) Lars
>
>
>
> In article <8gtdbo$2mh$1 at peque.uv.es>, "Jordi Pérez-Tur"
> <jpereztur at ibv.csic.es> wrote:
> >Hi,
> >
> >    I would be interested in protocols for amplifiying short
> areas of the
> >human genome (about 200bp) with a high GC content. So far we
> have been quite
> >unsuccesful with traditional profiles.
> >
> >    Thanks to all
> >
> >    Jordi Perez Tur
> >    jpereztur at ibv.csic.es
> >
> >
> >
> >
>
>
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