pcr protocol

ParentE at dfo-mpo.gc.ca ParentE at dfo-mpo.gc.ca
Tue May 30 10:40:12 EST 2000


Hi

It is usually not a problem to amplify 200bp. Could you give us more details
about the conditions? Are you sure of youre primers?

Betaine should help you with high GC.



Eric Parent
Maurice Lamontagne Institute
Fisheries and Oceans Canada
Invertebrates and Experimental Biology
850 route de la mer, P.O. box. 1000
Mont-Joli, Qc	
Canada	
G5H 3Z4

parente at dfo-mpo.gc.ca
> http://www.qc.dfo-mpo.gc.ca/iml/fr/intro.htm
> 
> 


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