RANDOM primer labelling efficiency

LoPony ctfrench at wwisp.com
Tue May 30 23:13:41 EST 2000

Depends on the template.  Sometimes not denaturing results in successful
labeling, but not always.  I wouldn't try it with another template and
expect to get the same results.

Take care.
Christopher Todd French
UAB Dept. of Comparative Medicine
1670 Univ. Blvd, VH 422
1530 3rd Ave. S
Birmingham, AL 35294-0019


"George Rutherford" <gruther at bilbo.bio.purdue.edu> wrote in message
news:gruther-1705001427160001 at arbpc3.btny.purdue.edu...
> In article <39221670.C3DC6CE0 at musica.mcgill.ca>, Mano
> <mdep at musica.mcgill.ca> wrote:
> > Hi all,
> > my collegue does not denature the DNA template before random primer
> > labelling and claims to get efficient incorporation of 32P-dCTP.  Is
> > denaturation of the template a "MUST"?  I always do , nevertheless.
> >
> I once knew a rookie grad student who couldn't get his RP rxns to work. I
> asked him if he was denaturing his template. He gave me a blank look which
> I took for a "no". His rxns started working.
> George

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