Error Prone PCR

LoPony ctfrench at wwisp.com
Tue May 30 23:30:33 EST 2000


Try using a mix of Vent polymerase (with the proofreading) and Taq.  The
problem with Vent is that it's difficult to produce a very long product
(even a couple of kb is big for it) and the problem with Taq is that it's
error prone.  A mix of the two works well many times.

Good luck.

--
==============================
Christopher Todd French
UAB Dept. of Comparative Medicine
1670 Univ. Blvd, VH 422
1530 3rd Ave. S
Birmingham, AL 35294-0019

==============================

"Simon Margerison" <simon.margerison at cambridgeantibody.com> wrote in message
news:392C0083.D9E694E3 at cambridgeantibody.com...
> Hi
>
> A colleague of mine is interested in performing some error prone pcr.
> Does anyone know any good methods or reagents for this. Any help would
> be appreciated, either by direct email to
>
> ruth.featherstone at camb-antibody.co.uk
>
> or on the board.
>
> Cheers
>
> Simon
>
> --
>
>   Simon Margerison
>   Cambridge Antibody Technology Ltd.
>   The Science Park,
>   Melbourn, Nr. Royston,
>   Cambs.
>   SG8 6JJ
>
>   Tel:+44 (0)1763 269281
>   Fax:+44 (0)1763 263413
>   mailto:simon.margerison at cambridgeantibody.com
>
>







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