non-radioactive method for detection of phosphorylated proteins

Frank O. Fackelmayer Frank.Fackelmayer at
Wed May 31 02:51:53 EST 2000

g wrote:
> Is there any non-radioactive alternative to gamma-P32 to detect
> phosphorylated proteins?
> Thanks!
> Barroso

Hi Barroso,
There are commercial antibodies against phospho-serine,
phospho-threonin, and phospho-tyrosine. The anti-phospho-tyrosine
antibody is used by quite a lot of people in signal transduction
research. I have no personal experience with this one (from the data I
have seen it seems to be ok) but the two others are not worth the money.


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