non-radioactive method for detection of phosphorylated proteins
Frank O. Fackelmayer
Frank.Fackelmayer at uni-konstanz.de
Wed May 31 02:51:53 EST 2000
> Is there any non-radioactive alternative to gamma-P32 to detect
> phosphorylated proteins?
There are commercial antibodies against phospho-serine,
phospho-threonin, and phospho-tyrosine. The anti-phospho-tyrosine
antibody is used by quite a lot of people in signal transduction
research. I have no personal experience with this one (from the data I
have seen it seems to be ok) but the two others are not worth the money.
More information about the Methods