How does G-50 work?

Dr. Duncan Clark Duncan at
Wed May 31 04:07:49 EST 2000

In article <8h0tde$in0$1 at>, kab35 at writes
>Could you please explain (I must  have a mental block) how G-50 allows
>linearized labeled riboprobe to pass through and how unlabelled
>nucleotides are trapped in the G-50 column?
>I understand size exclusion must play a role,

Can I suggest you talk nicely to Amersham Pharmacia Biotech, in fact I
suggest one person in every lab asks them nicely, for their principles
and methods handbooks on the following:

Hydrophobic Interaction Chromatography - Cat. No. 18-1020-90
Ion Exchange Chromatography - Cat. No. 18-1022-19
Gel filtration - Cat. No. 18-1022-18.

Great handbooks and they used to be free although I see they now have a
UKP5.00 price tag in the catalogue.

The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382

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