Long range PCR
clarosa at biocomp.unl.edu
Wed May 31 17:18:41 EST 2000
> We would like to make a 16 kb PCR. Anybody had such an experience on
> this ? Any suggestions in terms of the enzymes, the reagents, and the
> machine ? What's the best thermo cycler to achieve this ?
> Thanks in advance.
Thermo cycler matters little... you need 8 min extension times. use a
error correcting taq or mix. There are several good commercial kits,
Epicenter Failsafe...Gibco,,, stratagene all have kits... and extensive
protocols with the kits.
Do a little web search you will find tons of protocols...
More information about the Methods