Phenol pH?

Ned Mantei mantei at cell.biol.ethz.ch
Wed Nov 1 01:51:16 EST 2000


In article <JIEHICBJDMGFLNGJIBDMIENGCAAA.alansmith at students.wisc.edu>, 
alansmith at students.wisc.edu ("Alan Smith") wrote:

>Could someone please describe to me how the chloroform can protect
>the DNA from being removed by the acidic phenol.

The addition of chloroform makes the organic phase considerably more 
hydrophobic, and I would expect this to affect the partitioning of DNA.

-- 
Ned Mantei
Department of Cell Biology, Swiss Federal Institute of Technology
CH-8093 Zurich, Switzerland






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