Phenol pH?

Ned Mantei mantei at
Wed Nov 1 01:51:16 EST 2000

alansmith at ("Alan Smith") wrote:

>Could someone please describe to me how the chloroform can protect
>the DNA from being removed by the acidic phenol.

The addition of chloroform makes the organic phase considerably more 
hydrophobic, and I would expect this to affect the partitioning of DNA.

Ned Mantei
Department of Cell Biology, Swiss Federal Institute of Technology
CH-8093 Zurich, Switzerland

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