Re-ECLing nitrocellose??

Neal Robert Melvin nrmelvin at
Wed Nov 1 04:14:58 EST 2000

Has anyone ever tried to 're-do' an ECL reaction on an NC membrane that
has been previously processed, and subsequently dried?? Do you have to
strip the membrane first, and re-apply primary and secondary antibodies
even if you want to re-probe with the same primary as you used


"If there are many universes, there will be one where there is a set of
numbers suitable to life... we are in that one"

Martin Rees, Astronomer Royal 

Neal Melvin
University of Calgary - Faculty of Medicine
Department of Neuroscience - Neuroscience Research Group (NRG)
Health Sciences Centre
3330 Hospital Drive, NW
Calgary, Alberta, Canada
T2N 4N1

e-mail: nrmelvin at
Phone: (403) 220-4490 (lab)
       (403) 220-7035 (office)
       (403) 283-8731 (fax)

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