Phenol pH?

Dr. Hiranya S. Roychowdhury hroychow at nmsu.edu
Wed Nov 1 22:23:26 EST 2000


Not really the reason. Extraction w/o ChCl3 does not yield lower amounts.
CHCl3 is added also to make the org phase heavier and aid in protein
denaturation.

At 07:51 AM 11/1/00 +0100, Ned Mantei wrote:
>In article <JIEHICBJDMGFLNGJIBDMIENGCAAA.alansmith at students.wisc.edu>, 
>alansmith at students.wisc.edu ("Alan Smith") wrote:
>
>>Could someone please describe to me how the chloroform can protect
>>the DNA from being removed by the acidic phenol.
>
>The addition of chloroform makes the organic phase considerably more 
>hydrophobic, and I would expect this to affect the partitioning of DNA.
>
>-- 
>Ned Mantei
>Department of Cell Biology, Swiss Federal Institute of Technology
>CH-8093 Zurich, Switzerland
>
>
>
Dr. Hiranya Sankar Roychowdhury
College Asst. Prof.
Molecular Biology,
Dept. of Chemistry & Biochemistry	
Box 30001 - 3MLS
New Mexico State University
Las Cruces, NM 88003

Lab: (505) 646 4722
Office: (505) 646 8256
hroychow at nmsu.edu


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