Problem of MTT assay!

Terry Lau Chi-Keung.Lau at polyu.edu.hk
Thu Nov 2 08:41:51 EST 2000


Hi,

Recently, I would like to use the MTT assay to analyse the effect of TNF
alpha in C6 cell proliferation. However, the result is so discouraging
that the control (without adding the TNF) would have a higher
proliferation rate than the experimental one! I have performed several
time for this experiment but the result is the same! Would anyone have
this experience or any precautions of doing MTT assay?!

Thanks in advance!

Terry!






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