Beckman sequencer problems

John Philo "jphilo*NO SPAM12*" at
Thu Nov 2 17:06:33 EST 2000


The best place for these sorts of issues is the Association for
Biotechnology Resource Facilities (ABRF) discussion list, where there is
a huge amount of sequencing expertise. Go to and you can
find instructions for how to subscribe and post.

'Hope this helps.

John Philo
Alliance Protein Laboratories

Ivo Rieu wrote:
> Hello, I would like to come in contact with people that are using a
> Beckman CEQ2000 sequencer for DNAseq.analysis.
> The problem we have here is that the result is very variable. If we do
> the same sequences multiple times, one may be very nice, the other crap.
> We have the feeling that we are working on the edge of what the
> sequencer can handle, so sometimes it is just allright, while the next
> time the sample we try to sequence is just out the range the sequencer
> can handle. And we don't know wether what is out of range is the amount
> of template, the salt or something else that is not optimal. The most
> observed thing that goes wrong is a unstable, decreasing current, and a
> very late loading of the sample on the column (what is cause and what is
> result?).
> - Any idea what we do wrong ?
> - Maybe someone has made some modifications to the original protocol
> that improve the sequencing ?
> - ??
> Ivo Rieu
> Dept. Plant Cell Biology
> University of Nijmegen
> The Netherlands
> irieu at

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