extracting dna from acrylamide gels

Moritz Hertel hertelmo at baba.ethz.ch
Fri Nov 3 05:36:48 EST 2000


I have no experience with acrylamid gels, but its very easy with Agarose gels.
Just stamp out some material with a "yellow" pipet tip, and give this littel
"gel worm" to your PCR reaction. Agarose will melt in the initial 94 degree
Step and will be to diluted if the PCR is over...I would suppose that it also
work with a 50 ul PCR and the smalest amount of Acrylamid gel you can handle.
Of course its a quick and dirty methode, but it if it works... :-)
good luck
Moritz






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