martin.offterdinger at akh-wien.ac.at
Fri Nov 3 06:42:50 EST 2000
"Frank Fuerst" <ffrank at rz.uni-potsdam.de> schrieb im Newsbeitrag
news:8ts15v$22ue$1 at fu-berlin.de...
> "Richard P. Grant" <rpg14 at yahoo.co.uk.invalid> wrote:
> >In article <8tq8il$8nq$1 at news.doit.wisc.edu>,
> >klenchin at facstaff.REMOVE_TO_REPLY.wisc.edu (Dima Klenchin) wrote:
> >> The nickel is gone. We are talking about _recharging_ and that
> >> necessarily implies stripping the original, fully or partially reduced,
> >From Cornelius' posting, that's not that crystal clear. I'm not sure
> >whether he reduced it in the nickel-bound state or not.
> I would even say that it _is_ clear that the nickel was still there.
> The original poster wanted an alternative for imidazole as an eluent,
> and asked wether EDTA or DTT would work:
> >We now want to turn to an alternative elution
> >approach e.g. EDTA. [...]
> >I have also been thinking about whether one could
> >use high concentrations of DTT. In the manual
> >(Quiaexpressionist) they write that DTT can reduce
> >Nickel thereby preventing nickel from binding His-tagged
> And Cornelius warned him to use DTT.
> Bye, Frank
What about dialysing out the imidazole?
More information about the Methods