Sv: blunt-end ligation

Dr. Duncan Clark Duncan at
Fri Nov 3 12:13:15 EST 2000

In article <30CM5.2554$n3.206692 at>, the eminent Jens
Tornoe at Mobilix Internet -- wrote
>If low temp is good for annealing and high temp is beneficial for the
>ligation, thermocycling could be an idea.

Been done and published in NAR as one of the single sheet methods at the
back maybe 3-4 years ago.

The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
GeneSys Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382

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