Sv: blunt-end ligation

Dr. Duncan Clark Duncan at nospam.demon.co.uk
Fri Nov 3 12:13:15 EST 2000


In article <30CM5.2554$n3.206692 at news0.mobilixnet.dk>, the eminent Jens
Tornoe at Mobilix Internet -- http://www.mobilixnet.dk/ wrote
>If low temp is good for annealing and high temp is beneficial for the
>ligation, thermocycling could be an idea.

Been done and published in NAR as one of the single sheet methods at the
back maybe 3-4 years ago.

Duncan
-- 
The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
GeneSys Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382
http://www.dnamp.com
http://www.genesys.demon.co.uk






More information about the Methods mailing list