Sv: blunt-end ligation
Dr. Duncan Clark
Duncan at nospam.demon.co.uk
Fri Nov 3 12:13:15 EST 2000
In article <30CM5.2554$n3.206692 at news0.mobilixnet.dk>, the eminent Jens
Tornoe at Mobilix Internet -- http://www.mobilixnet.dk/ wrote
>If low temp is good for annealing and high temp is beneficial for the
>ligation, thermocycling could be an idea.
Been done and published in NAR as one of the single sheet methods at the
back maybe 3-4 years ago.
The problem with being on the cutting edge is that you occasionally get
sliced from time to time....
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