histag elution alternative

[Richard P. Grant]

In article <8u1k5d$fuq$1 at news.doit.wisc.edu>, 
klenchin at facstaff.REMOVE_TO_REPLY.wisc.edu (Dima Klenchin) wrote:

>  Now the only thing that remains is to remove this Ni+ and
> recharge with Ni2+. Surprisingly, it turned out to be _very_ difficult:

So it looks as if reduced nickel binds the column, really rather 
strongly?

-- 
Richard P. Grant                                      MRC Lab of Mol Biol
rpg 'at' mrc-lmb.cam.ac.uk    http://www2.mrc-lmb.cam.ac.uk/personal/rpg/
                         'I have detailed files'