histag elution alternative

[Michael L. Sullivan]

>
>I would expect that it is Ni°-metal, which is of course poorly soluble
>in anything, and which sticks to the column material because upon
>reduction, the metal starts crystallizing there, not in the free
>solution.

I think you are right here.  My freshman chemistry texts says "nickel
exhibits an oxidation state of +2 almost exclusively".  I'd be surprised
then that it would be so easy to make +1 nickel simply by treating with DTT.

Mike

Michael L. Sullivan, Ph.D

U.S. Dairy Forage Research Center
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Madison WI, 53706

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