Visualization of 16S rRNA

jph at ibt.dtu.dk jph at ibt.dtu.dk
Mon Nov 6 10:11:10 EST 2000


Hi.

Why can I see a speam from a 4 µg 16S rRNA standard sample on a TAE gel and 
nothing on a denaturing formaldehyde gel. The samples were prepares in the 
same way for both gels ie. same chemicals, formaldehyde, formamide, MOPS, 
load buffer.

EtBr was added to the run buffer. Does it penetrate the formaldehyde gel less 
well? Soaking the gell in DEPC water efter the run does not help.

Is the MOPS a good food source for bacteria ie. leading to RNases.

Best regards

Jacob Hofman-Bang






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