extracting dna from acrylamide gels
Rick A. Bright
rbright at emory.edu
Mon Nov 6 22:41:00 EST 2000
Gene Clean Spin kits are excellent for this purpose.
"Jeffrey Markert" <markertNOSPAM at mbcl.rutgers.edu> wrote in message
> I'm looking for advice on extracting DNA bands from acrylamide gels. My
> goal is to recover enough DNA for a few PCR reactions. The acrylamide gels
> are stained in EtBr and were made with FMC's Long Ranger mix.
> I've been using the ammonium acetate elution protocol from Maniatis, but
> I'm not finding it to be very reliable in my hands and it would be nice to
> find a procedure that doesn't require an o/n incubation.
> I'm wondering whether there's a simpler and quicker way that will work for
> PCR. Do acrylamide or EtBr inhibit PCR? Could I just elute the gel slice
> in water to avoid having to get rid of the ammonium acetate? Could I just
> freeze (or boil) the gel slice? Any thoughts or anecdotes are welcome.
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