making a master mix for SDS-PAGE

Michael L. Sullivan mlsulliv at facstaff.wisc.edu
Tue Nov 7 10:27:02 EST 2000


Since I mostly run 10% SDS-PAGE gels, is there any reason I can't make up a
master mix of acrylamide, water, SDS, and buffer that would need only to
have APS and TEMED added?

I know that such mixes are available commercially, but I wasn't sure
whether those might have some additive that would prevent some dire
consequence of mixing the other components for longer term storage and use.

Thanks for any insight.

Mike

Michael L. Sullivan, Ph.D

U.S. Dairy Forage Research Center
1925 Linden Drive West
Madison WI, 53706

(608) 264-5144 Phone
(608) 264-5147 Fax


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