Loss of expression, help!

Dr. Duncan Clark Duncan at nospam.demon.co.uk
Tue Nov 7 10:40:51 EST 2000

In article <NDBBKMADGLKJLODKIFGAEEOMCAAA.wind at biobase.dk>, the eminent
Troels Wind at BIOSCI/MRC Human Genome Mapping Project Resource Centre
>It is not a question of codon-choice; all codons are selected for optimal

Secondary structure i.e. big hairpin in the mRNA of the second

One of the additional sequences makes your protein lethal? 

The second construct is re-arranging or kicking the plasmid out as it

I presume sequencing shows the 2nd construct is correct with no
accidental change of reading frame etc. 


The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
GeneSys Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382

More information about the Methods mailing list