Loss of expression, help!
Dr. Duncan Clark
Duncan at nospam.demon.co.uk
Tue Nov 7 10:40:51 EST 2000
In article <NDBBKMADGLKJLODKIFGAEEOMCAAA.wind at biobase.dk>, the eminent
Troels Wind at BIOSCI/MRC Human Genome Mapping Project Resource Centre
>It is not a question of codon-choice; all codons are selected for optimal
Secondary structure i.e. big hairpin in the mRNA of the second
One of the additional sequences makes your protein lethal?
The second construct is re-arranging or kicking the plasmid out as it
I presume sequencing shows the 2nd construct is correct with no
accidental change of reading frame etc.
The problem with being on the cutting edge is that you occasionally get
sliced from time to time....
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