making a master mix for SDS-PAGE

clarosa clarosa at biocomp.unl.edu
Tue Nov 7 12:24:04 EST 2000


I think it is not done this way because it does not work... otherwise,, we
would be doing it this way.

"Michael L. Sullivan" wrote:

> Since I mostly run 10% SDS-PAGE gels, is there any reason I can't make up a
> master mix of acrylamide, water, SDS, and buffer that would need only to
> have APS and TEMED added?
>
> I know that such mixes are available commercially, but I wasn't sure
> whether those might have some additive that would prevent some dire
> consequence of mixing the other components for longer term storage and use.
>
> Thanks for any insight.
>
> Mike
>
> Michael L. Sullivan, Ph.D
>
> U.S. Dairy Forage Research Center
> 1925 Linden Drive West
> Madison WI, 53706
>
> (608) 264-5144 Phone
> (608) 264-5147 Fax
>
> ---






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