making a master mix for SDS-PAGE
klenchin at facstaff.REMOVE_TO_REPLY.wisc.edu
Tue Nov 7 12:37:59 EST 2000
In article <email@example.com>,
mlsulliv at facstaff.wisc.edu ("Michael L. Sullivan") wrote:
:Since I mostly run 10% SDS-PAGE gels, is there any reason I can't make up a
:master mix of acrylamide, water, SDS, and buffer that would need only to
:have APS and TEMED added?
There is no reason not to do it if you only need one final AA
concentration. Absolutely no problem. Just remember
to store acrylamide in the dark bottle (wrapped in foil is better) and
not for too long.
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