making a master mix for SDS-PAGE

Dima Klenchin klenchin at facstaff.REMOVE_TO_REPLY.wisc.edu
Tue Nov 7 12:37:59 EST 2000


In article <v04011700b62dcef7478a@[144.92.64.179]>, 
mlsulliv at facstaff.wisc.edu ("Michael L. Sullivan") wrote:
:Since I mostly run 10% SDS-PAGE gels, is there any reason I can't make up a
:master mix of acrylamide, water, SDS, and buffer that would need only to
:have APS and TEMED added?

There is no reason not to do it if you only need one final AA 
concentration. Absolutely no problem. Just remember 
to store acrylamide in the dark bottle (wrapped in foil is better) and
not for too long. 

        - Dima






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