making a master mix for SDS-PAGE

Helen Niederwald helennie at
Tue Nov 7 13:07:28 EST 2000

In article <v04011700b62dcef7478a@[]>,
  mlsulliv at ("Michael L. Sullivan") wrote:
> Since I mostly run 10% SDS-PAGE gels, is there any reason I can't
make up a
> master mix of acrylamide, water, SDS, and buffer that would need only
> have APS and TEMED added?

I have been doing this for years. Works fine. I just keep it in the
Usually lasts me a couple of weeks (4-8).


> I know that such mixes are available commercially, but I wasn't sure
> whether those might have some additive that would prevent some dire
> consequence of mixing the other components for longer term storage
and use.
> Thanks for any insight.
> Mike
> Michael L. Sullivan, Ph.D
> U.S. Dairy Forage Research Center
> 1925 Linden Drive West
> Madison WI, 53706
> (608) 264-5144 Phone
> (608) 264-5147 Fax
> ---

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