making a master mix for SDS-PAGE

Helen Niederwald helennie at my-deja.com
Tue Nov 7 13:07:28 EST 2000


In article <v04011700b62dcef7478a@[144.92.64.179]>,
  mlsulliv at facstaff.wisc.edu ("Michael L. Sullivan") wrote:
> Since I mostly run 10% SDS-PAGE gels, is there any reason I can't
make up a
> master mix of acrylamide, water, SDS, and buffer that would need only
to
> have APS and TEMED added?


I have been doing this for years. Works fine. I just keep it in the
fridge.
Usually lasts me a couple of weeks (4-8).

Helen.




>
> I know that such mixes are available commercially, but I wasn't sure
> whether those might have some additive that would prevent some dire
> consequence of mixing the other components for longer term storage
and use.
>
> Thanks for any insight.
>
> Mike
>
> Michael L. Sullivan, Ph.D
>
> U.S. Dairy Forage Research Center
> 1925 Linden Drive West
> Madison WI, 53706
>
> (608) 264-5144 Phone
> (608) 264-5147 Fax
>
> ---
>


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