e. coli lysis

R. John Lye rjl6n at virginia.edu
Tue Nov 7 15:56:42 EST 2000


Mark Bowen wrote:

> I know of many other bug-busting  methods, but
> was wondering if people had experience getting reproducible lysis
> without affecting LacZ activity.

How about lysozyme treatment followed by sonication?  That's what I
use to purify fusion proteins.

John






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