how to quantify pcr products

John Thompson jrt at home.com
Tue Nov 7 19:45:53 EST 2000


Karen Roberts <leehrat at mindspring.com> wrote:

>I have tried using spec measurements but the yield is so low after
>I have purified the products that the readings give me next to or
>nothing.
>
Try Sybr Green dyes from molecular probes.  Sensitive down in the ng
range with a microtiter plate assay and a good microplate fluormeter.
Yours,
John Thompson






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