SOD assay kit
Paul S. Brookes.
brookes at uab.edu
Wed Nov 8 09:35:16 EST 2000
Why bother with a kit? The original SOD paper by Fridovitch & McCord has
a perfectly good method. It's based on the inhibition (by SOD) of the
reduction of cytochrome c in a standardized xanthine/xanthine oxidase
system. As I recall, 1 unit of SOD is the amount that inhibits the rate by
half. Its an easy assay in a spec' at 550nm. Don't forget to add cyanide
too, to inhibit re-oxidation of the cyt-c by mitochondrial cytochrome
oxidase. However, not too much cyanide or it will inhibit Cu/Zn SOD aswell
- which happens to be a good way of measuring MnSOD activity.
Dr. Paul S. Brookes. (brookes at uab.edu)
UAB Department of Pathology, G004 Volker Hall
1670 University Blvd., Birmingham AL 35294 USA
Tel (001) 205 934 1915 Fax (001) 205 934 1775
The quality of e-mails can go down as well as up
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