making a master mix for SDS-PAGE

Kevin A. Morano kevin.a.morano at uth.tmc.edu
Wed Nov 8 15:01:19 EST 2000


No problem.  Works just fine, dark or light bottle.  Good at least 2 
months.  I also use a 25% APS stock also good for months - don't make it 
fresh.  I am impatient, so I do a rapid polymerization:

10 ml gel mix (10%)
100 ul 25% APS
10 ul TEMED

Pour, overlay with water, come back 5 minutes later.  Ready to go.

Kevin






More information about the Methods mailing list