PCR SMEARING

[Philip O'Brien]

We have been getting a lot of smearing in our PCR reactions.  Sometimes we
get a discreet band, but most of the time its a smear.  We get this with
different primer pairs so it dosent't appear to be confined to one pair.
The reactions are all set up on ice to minimize aberrant priming before
thermalcycling.  I would be grateful if anyone could give me information on
how to eliminate the problem.


Phil

Philip O'Brien
Division of Science
School of Biological Sciences & Biotechnology ,
Murdoch University,
Murdoch WA 6150,
AUSTRALIA

Ph Nat     61-8-9360 2785
Fax         61-8-9310 7084
http://www.murdoch.edu.au/home.html

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