flow cytometry adherent cells
Wolfgang.Schechinger at med.uni-tuebingen.de
Thu Nov 9 08:41:16 EST 2000
Since it's probably important for you to see what is happening inside
single cells, the best solution might be to use a fluorescence
microscope coupled to a sensitive CCD camera for real time imaging.
With FACS, you only might see an average cytosolic concentration.
> I am trying to measure calcium flux in an adherent cell line with
> either Fura2 or indo 1. Does anyone know of a good way to do this.
> I should tell you I have never done flow before.
> Adam Biales
> University of Cincinnati
This message is encrypted! Use your brain to decode it.
Dr. Wolfgang Schechinger, Dept. of Pathobiochemistry
University of Tuebingen, Germany
email: wolfgang.schechinger at med.uni-tuebingen.de
usual disclaimers apply
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