flow cytometry adherent cells

[Wolfgang Schechinger]

Adam, 

Since it's probably important for you to see what is happening inside 
single cells, the best solution might be to use a fluorescence 
microscope coupled to a sensitive CCD camera for real time imaging.

With FACS, you only might see an average cytosolic concentration.

Wolfgang

> I am trying to measure calcium flux in an adherent cell line with
> either Fura2 or indo 1.  Does anyone know of a good way to do this. 
> I should tell you I have never done flow before.
> 
> Adam Biales
> University of Cincinnati
> 
> 
-----
This message is encrypted! Use your brain to decode it.
-----
Dr. Wolfgang Schechinger, Dept. of Pathobiochemistry
University of Tuebingen, Germany
email: wolfgang.schechinger at med.uni-tuebingen.de 
wwWait: http://www.medizin.uni-tuebingen.de/~wgschech/start.htm
-----
usual disclaimers apply 
-----


---