removal of SDS from a protein

Michael L. Sullivan mlsulliv at facstaff.wisc.edu
Thu Nov 9 11:19:29 EST 2000


If a protein has been denatured with SDS, will extensive dialysis against
buffer lacking SDS remove the detergent that is bound to the protein?  Or
would one have to get rid of the SDS by adding other agents to the dialysis
buffer, like triton-X or ethanol (I think I've seen reference to this)?

Thanks for any input.

Mike

Michael L. Sullivan, Ph.D

U.S. Dairy Forage Research Center
1925 Linden Drive West
Madison WI, 53706

(608) 264-5144 Phone
(608) 264-5147 Fax


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