Qiagen Buffer Receipes
Richard P. Grant
rpg14 at yahoo.co.uk.invalid
Fri Nov 10 03:59:35 EST 2000
In article <18.104.22.168.20001109203923.007c2ba0 at cnmailsvr.nmsu.edu>,
hroychow at nmsu.edu ("Dr. Hiranya S. Roychowdhury") wrote:
> I believe the N3 is the 3M KOAc and the PB is similar to PE with a higher
> salt conc.
N3 is probably lower than 3M KOac as I know one can get away with a lot
less. Might be as low as 300 mM. PE will be (probably) Tris-buffered
KOAc (agh, don't quite me on that (-: - the concentration is reasonably
low, anyway, about 50 mM final ISTR).
PB is about 4M GnHCL/50% isopropanol (sniff it). This is all published
- but might take some finding.
> Wont the Qiagen people let us know the recipe, since we
> invariably run out of the solns before using up all the columns?
We usually run out of columns first . . . . well, the mini-prep spin
columns anyway, we don't use the maxi/midi prep gear.
Richard P. Grant MRC Lab of Mol Biol
rpg 'at' mrc-lmb.cam.ac.uk http://www2.mrc-lmb.cam.ac.uk/personal/rpg/
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