Qiagen Buffer Receipes

Richard P. Grant rpg14 at yahoo.co.uk.invalid
Fri Nov 10 04:00:26 EST 2000

In article < at cnmailsvr.nmsu.edu>, 
hroychow at nmsu.edu ("Dr. Hiranya S. Roychowdhury") wrote:

> I believe the N3 is the 3M KOAc and the PB is similar to PE with a higher
> salt conc. 

N3 is probably lower than 3M KOac as I know one can get away with a lot 
less.  Might be as low as 300 mM. PE will be (probably) Tris-buffered 
KOAc (agh, don't quite me on that (-: - the concentration is reasonably 
low, anyway, about 50 mM final ISTR - oh and some EDTA, probably).

PB is about 4M GnHCL/50% isopropanol (sniff it).  This is all published 
- but might take some finding.

> Wont the Qiagen people let us know the recipe, since we
> invariably run out of the solns before using up all the columns?

We usually run out of columns first . . . .  well, the mini-prep spin 
columns anyway, we don't use the maxi/midi prep gear.



Richard P. Grant                                     MRC Lab of Mol Biol
rpg 'at' mrc-lmb.cam.ac.uk   http://www2.mrc-lmb.cam.ac.uk/personal/rpg/
        -- 'An angel has quite a large carrying capacity' --

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