Buffer Receipes
Armando Magrelli
amagrelli at ibc.rm.cnr.it
Fri Nov 10 04:47:25 EST 2000
>We are searching original or identical buffer receipes (PB, N3 etc.) of
>the QIAGEN products, which were not available in the instruction
>manuals.
>
>Thanks!
>
>Andreas
BUFFER P1: 50mM Tris-Cl pH 8.0
10 mM EDTA
100 ug/ml RNAse A
P2: 200 mM NAOH, 1% SDS
P3 or N3: 3.0 M potassium acetate, pH 5.5
Buffer FWB (QIAfilter Wash Buffer): 750 mM NaCl
50 mM MOPS,pH 7.0
15% isopropanol
Buffer QBT (Equilibration Buffer): 750 mM NaCl
50 mM MOPS,pH 7.0
15% isopropanol
0,15% Triton X-100
Buffer QC (Wash Buffer): 1.0 M NaCl
50 mM MOPS,pH 7.0
15% isopropanol
Buffer QF (Elution Buffer) 1,25 M NaCl
50mM Tris-Cl pH 8.5
15% isopropanol
Buffer QN (Elution Buffer) 1.6 M NaCl
50 mM MOPS,pH 7.0
15% isopropanol
TE 10 mM Tris-Cl pH 8.0
1 mM EDTA
STE 100 mM NaCl
10 mM Tris-Cl pH 8.0
1 mM EDTA
Hope this Help
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