Cre/lox and hyphal fungi questioin

Chris Boyd cboyd at holyrood.ed.ac.uk
Fri Nov 10 06:34:58 EST 2000


Dirk Müller <d.mueller at lb.tu-berlin.de> wrote:
: i wonder if someone has ever tested to use the cre/lox system in hyphal
: fungi especially in the genus Aspergillus. For homologous transformation in
: Aspergillus one needs a real large homolog sequence stretch so i wonder if
: it makes sense and is possible to use cre/lox???

Well it's been done in the rice blast fungus Magnaporthe grisea.  My
guess is that Aspergillus would also work.  See

1. Choi, S., Begum, D., Koshinsky, H., Ow, D. W. and Wing, R. A. (2000)
   `A new approach for the identification and cloning of genes:  the
   pBACwich system using Cre/lox site-specific recombination.' Nucleic
   Acids Res., 28, e19.

(Medline 10710436)

Best wishes,
-- 
Chris Boyd                      | from, but \    Medical Genetics Section
Chris.Boyd at ed.ac.uk             | not for,  /    MMC, Edinburgh Uni.,
http://www.ed.ac.uk/~cboyd                       EH4 2XU, SCOTLAND






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