Help!!!Restriction enzyme digestion troubles.

Rodney Pettway rodneypettway at hotmail.com
Fri Nov 10 09:05:11 EST 2000


I start out with high molecular weight DNA.  When I try and carryout my digestion it seems as though my DNA is being degraded by the enzyme and I have tried two different enzymes.  When I just add water or buffer or water/buffer to the DNA it is fine i.e. high molecular weight.  what should I do!!!!!!
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