Help!!!Restriction enzyme digestion troubles.

Rodney Pettway rodneypettway at
Fri Nov 10 09:05:11 EST 2000

I start out with high molecular weight DNA.  When I try and carryout my digestion it seems as though my DNA is being degraded by the enzyme and I have tried two different enzymes.  When I just add water or buffer or water/buffer to the DNA it is fine i.e. high molecular weight.  what should I do!!!!!!
-------------- next part --------------
An HTML attachment was scrubbed...

More information about the Methods mailing list