Help!!!Restriction enzyme digestion troubles.
Helen Niederwald
helennie at my-deja.com
Fri Nov 10 10:29:21 EST 2000
In article <LAW2-OE458M19VMczi9000001bf at hotmail.com>,
rodneypettway at hotmail.com ("Rodney Pettway") wrote:
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> I start out with high molecular weight DNA.
What kind? Plasmid? Chromosomal? What source? What purification method?
When I try and carryout my d=
> igestion it seems as though my DNA is being degraded by the enzyme
What else do you expect? It's a restriction enzyme. If you digest
chromosomal DNA you will see a smear.
and I =
> have tried two different enzymes. When I just add water or buffer or
wat=
> er/buffer to the DNA it is fine i.e. high molecular weight. what
should =
> I do!!!!!!
Test the enzyme on a standard template. However, most likely nothing is
wrong with your experiment. More likely, your interpretation needs some
refinement.
Regards,
Helen.
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> <HTML><BODY STYLE=3D"font:10pt verdana; border:none;"><DIV>I start
out wi=
> th high molecular weight DNA. When I try and carryout my
digestion =
> it seems as though my DNA is being degraded by the enzyme and I have
trie=
> d two different enzymes. When I just add water or buffer or
water/b=
> uffer to the DNA it is fine i.e. high molecular weight. what
should=
> I do!!!!!!</DIV></BODY></HTML>
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