ELISA with bacterially expressed protein

Arnoud van Vliet avvliet at knoware.nl
Sat Nov 11 16:50:08 EST 2000

> I want do an ELISA with a (Drosophila) protein expressed in bacteria.
> according to the protocol the samples are READY FOR ELISA at this point.
> But unfortunately nothing is said about how to dilute the samples. Or
> should I use them undiluted?


each ELISA is different, and has to be optimized. You will have to titrate
your antigen batch. Also, you are not saying how you expressed it (high,
medium or low level), and what you want to do with it.

But in general, you will have to dilute it quite a bit, as ELISAs tend to be
quite sensitive

hope this helps

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