Westerns; napthol reagent etc

engelbert_buxbaum4 at my-deja.com engelbert_buxbaum4 at my-deja.com
Mon Nov 13 00:31:40 EST 2000


In article <200011121411.PAA19182 at intwww.zit.med.uni-tuebingen.de>,
  Wolfgang.Schechinger at med.uni-tuebingen.de ("Wolfgang Schechinger")
wrote:
> Hi all,
> Nickel enhances HRP in DAB staining. Actually it also should do it
> in ECL. Or is it a quencher?

The pigment produced by HRP from DAB is able to reduce Ni2+ (or Co2+) to
the element. Thus a brownish, light sensitive and fading pigment is
converted to a black, stable band. In other words, the Ni2+ is not
involved in the HRP reaction itself.

I have never tried what happens when Ni2+ is added to the luminol based
reaction mix used for chemoluminescence. My guess would be that there is
either no effect at all, or that there is quenching. But you could
easily try!


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