DEPC treatment of ethanol

Frank Ludewig frank.ludewig at uni-koeln.de
Mon Nov 13 03:29:21 EST 2000


You should NOT autoclave 100% ethanol!!!
mix DEPC-treated water and ethanol (p.a.) to yield 70 % ethanol.
Bye, FrankL


--
~~~~~~~~~~~~~~~~~~~~~~~!

Dr. Frank Ludewig
Department of Botany II
University of Cologne
Gyrhofstrasse 15
50931 Cologne
Germany

email: frank.ludewig at uni-koeln.de
Fax:   49-(0)221-4705039
Phone: 49-(0)221-4703388

http://www.uni-koeln.de/math-nat-fak/botanik/bot2/agflue/




Ali El-Salanti <isl40117 at worldonline.dk> schrieb in im Newsbeitrag:
8uo79e$r90$1 at news.net.uni-c.dk...
> Is it necessary to DEPC treat 70 and 100% ethanol prior to RNA
> precipitation. If yes how do you autoclave 100% ethanol.
> Thanks
> AS
>
>







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