DEPC treatment of ethanol

Ali El-Salanti isl40117 at worldonline.dk
Mon Nov 13 06:33:53 EST 2000


So when I make buffer I do not have to DEPC treat it as long as I use DEPC
water? But what if the things I use are not "ultra pure - high grade RNAse
free" fx EDTA or sodium acetate?
Thanks
Ali

"marianne carter" <mcarter3 at optusnet.com.au> wrote in message
news:3a0fc68b$0$19422$7f31c96c at news01.syd.optusnet.com.au...
> NO
> Same as you dont DEPC treat other solutions you make up eg EDTA
> etc........you use DEPC treated water only as it breaks specific chemical
> bonds ie heat treatment of some chemical solutions for use inn RNA
> purification.
>
>
> "Ali El-Salanti" <isl40117 at worldonline.dk> wrote in message
> news:8uo79e$r90$1 at news.net.uni-c.dk...
> > Is it necessary to DEPC treat 70 and 100% ethanol prior to RNA
> > precipitation. If yes how do you autoclave 100% ethanol.
> > Thanks
> > AS
> >
> >
>
>







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