DNAse 1 treatment of RNA samples

Ali El-Salanti isl40117 at worldonline.dk
Mon Nov 13 06:59:12 EST 2000


Hello.
I would like to hear your opinion on conditions for DNAse treatment of RNA
samples.
Some (Ambion+Promega) recommend that you DNAse 1 treat you sample at 37
degrees for at least 30 min. And if this doesn't work extend the incubation
time.
LifeTech. recommends to incubate at roomtemp. for no more than 15 minutes?
Does it depend on the buffer or what?
AS







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